Analysis of synaptic events in intracellular voltage clamp recordings
نویسندگان
چکیده
This course module introduces the analysis of data from intracellular patch-clamp recordings in the voltage clamp mode, focusing on the detection and statistical description of spontaneous as well as evoked post-synaptic currents (PSCs). After having completed the module, students should be able to detect PSCs by setting appropriate thresholds and analyze amplitude distribution, reliability and amplitude variability of the detected PSCs. Data from whole-cell patch-clamp recordings from layer-V pyramidal cells of rat somatosensory cortex are provided, where spontaneously occurring PSCs are used to train detection and assessment of amplitude distribution, while PSCs evoked via glutamate uncaging at presynaptic sites are used for reliability and amplitude variability measures.
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